base modifications

What Techniques are Used to Study Base Modifications?

Several advanced techniques are employed to study base modifications:
1. Bisulfite Sequencing: Used to detect DNA methylation by converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged.
2. Chromatin Immunoprecipitation (ChIP): Identifies protein-DNA interactions and histone modifications by precipitating DNA-bound proteins.
3. Mass Spectrometry: Can detect and quantify a variety of base modifications in both DNA and RNA.

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