There are several methods to create 3D tumor models:
1. Scaffold-Based Methods: These involve the use of natural or synthetic materials to provide a support structure for cancer cells to grow. Examples include hydrogels and biopolymers. 2. Spheroid Formation: Cancer cells are cultured to form spheroids, which are small, spherical clusters of cells that mimic the 3D structure of tumors. 3. Organoids: Derived from stem cells or primary tumor samples, organoids are miniaturized and simplified versions of organs that can be used to study tumor biology and drug responses. 4. Organ-on-a-Chip: This technology uses microfluidic devices to create a microenvironment that mimics the physiological conditions of human organs, including tumors.
