What are the Best Practices for Read Depth Normalization in Cancer Studies?
To achieve optimal results, researchers should follow these best practices:
1. Quality Control: Perform rigorous quality control to identify and mitigate technical biases. 2. Appropriate Method Selection: Choose normalization methods suited to the specific characteristics of the data. 3. Consistency: Apply consistent normalization techniques across all samples to ensure comparability. 4. Validation: Validate findings using independent datasets or orthogonal methods to confirm the robustness of the results.
