What Techniques Are Used to Detect Structural Variants?
Several advanced techniques are used to detect SVs in cancer: 1. Next-Generation Sequencing (NGS): High-throughput sequencing methods can identify SVs with high resolution. 2. Comparative Genomic Hybridization (CGH): This technique compares cancer DNA with normal DNA to identify copy number variations. 3. Fluorescence In Situ Hybridization (FISH): Uses fluorescent probes to detect specific DNA sequences on chromosomes, useful for identifying translocations. 4. Polymerase Chain Reaction (PCR): Can be employed to detect known SVs with high specificity.
